Introduction: The microarray has become a new research tool for technicians looking to view and interpret genes. It is essentially a merge of genomics, computer science, and nanotechnology. It allows for a detection of patterns or changes in transcription in normal and abnormal cells. For example, you can compare cancerous and normal cells easily. Basically, a DNA microarray chip is a solid matrix, like a glass slide, that is imprinted with arranged pattern of spots that each represent part of a genome.
Procedure: 1)Prepare the simulated microarray slide
*put drop appropriate gene solution onto each slide
2) Hybridized your microarray with labeled cDNAs from normal lung tissue and lung cancer tissue
*label normal with blue dye, cancerous with red dye
*drop 20 uL of hybridization solution onto each spot
3)Visualize your labeled mircroarray results
Results:
