Gene Disease Testing

Introduction:

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The genome is our hereditary code made up of DNA. Biotechnology uses this knowledge to manipulate organisms. Polymerase Chain Reaction is used to amplify a specific sequence of DNA.

P glo

The goal of this lab is to make bacteria glow. We will be inserting the DNA for Green Florescent Protein (GFP) into the bacteria's DNA. This will be done by subjecting the bacteria to heat shock. Heat shock is a rapid change in temperature. The rapid change in temperature will cause the bacteria to absorb DNA mediately around it, thus absorbing the DNA with the GFP gene. To ensure that only the bacteria that absorbed the DNA survive, we will attach the coding for the GFP with the coding for ampicilin resistance. Then, after the bacteria has gone through heat shock and absorbed the DNA, the bacteria then will be subjected to ampicillin, therefore killing off all of the bacteria that did not absorb the DNA. The remaining ampicillin resistant bacteria should glow green if all went correctly.

Identifiying Devil Crops: The study of GMOs

Well, the problem here with the GMOs has been grounded in history for many years. Actually, only as many years as GMOs have existed, which is only about 24, since they mainly started in 1986. But, nonetheless, GMO's are genetically modified organisms. Scientists have deemed it necessary to tinker with God's almighty creations in order to protect crops from frost, or disease. They manipulate the genes of species in order to breed specific, beneficial traits. However, many sensible people object to gmos because their effect on humans are unknown.


Procedure:
Grind fruit with pestle. Label the tubes 1 through 6. Add the master mix. place the the mixture in the PCR tubes. Place the PCR tubes in the thermal cycler. Put loading dye in each one. Run each one through gel electrophoresis.

Results/Discussion:
Lane 1: Test Food 1 Plant Master Mix
Lane 2: Test Food 1 GMO Master Mix
Lane 3: Test Food 2 Plant Master Mix
Lane 4: Test Food 2 GMO Master Mix


Lane 5: GMO+ Plant Master Mix
Lane 6: GMO+ GMO Master Mix
Lane 7: Gel Marker


However, our results might be skewed for we mixed up lanes 1 and 3 making our results different from what was expected




Lane 7: PCR Molecular weight ruler.