P glo

The goal of this lab is to make bacteria glow. We will be inserting the DNA for Green Florescent Protein (GFP) into the bacteria's DNA. This will be done by subjecting the bacteria to heat shock. Heat shock is a rapid change in temperature. The rapid change in temperature will cause the bacteria to absorb DNA mediately around it, thus absorbing the DNA with the GFP gene. To ensure that only the bacteria that absorbed the DNA survive, we will attach the coding for the GFP with the coding for ampicilin resistance. Then, after the bacteria has gone through heat shock and absorbed the DNA, the bacteria then will be subjected to ampicillin, therefore killing off all of the bacteria that did not absorb the DNA. The remaining ampicillin resistant bacteria should glow green if all went correctly.